Journal: Renal Failure

Article Title: miR-15a-5p suppresses peritoneal fibrosis induced by peritoneal dialysis via targeting VEGF in rats

doi: 10.1080/0886022x.2020.1811123

Figure Lengend Snippet: Figure 1. The rat uremia model was successfully constructed. (A) The serum of rats in each group was collected before periton- eal dialysis. The levels of creatinine (Scr) and blood urea nitrogen (BUN) are shown (p < 0.05). (B) Expression of miR-15a-5p and VEGF in the peritoneum, as detected by quantitative real-time PCR (qRT-PCR). p < 0.05 versus control, #p < 0.05 versus PD.

Article Snippet: Immunohistochemical staining of peritoneum tissue sections was performed using a rabbit two-step detection kit and a DAB developing kit (Goldenbridge Biotechnology Co. Ltd., Beijing, China).

Techniques: Construct, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control

Journal: Nature communications

Article Title: Circadian clock molecule REV-ERBα regulates lung fibrotic progression through collagen stabilization.

doi: 10.1038/s41467-023-36896-0

Figure Lengend Snippet: Fig. 1 | Decreased REV-ERBα protein abundance and increased protein levels of COL1A1 and LOX in IPF lungs compared to healthy control. Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc. Healthy controls contained 100% normal lung architecture with 85% alveoli surface area. IPF patient samples contained at least 50% lesion surface area. The protein abundance of REV-ERBα, COL1A1, and LOX were visualized and

Article Snippet: Compared to the control groups, the protein abundanceof REV-ERBαwas decreased in the healthy area (Control: 21.294%vs. healthy area from IPF: 11.296%) Fig. 1 | Decreased REV-ERBα protein abundance and increasedprotein levels of COL1A1 and LOX in IPF lungs compared to healthy control.Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc.

Techniques: Quantitative Proteomics, Control

Journal: Nature communications

Article Title: Circadian clock molecule REV-ERBα regulates lung fibrotic progression through collagen stabilization.

doi: 10.1038/s41467-023-36896-0

Figure Lengend Snippet: Fig. 7 | IAV infection induced dysregulation of profibrotic progression exa- cerbated in Rev-erbα Het mice. WT and Rev-erbα Het mice (equal number of male and female) infected (103 PFU/mouse) with IAV for 15 days, and lungs were sepa- rated for RNA/protein isolation, or fixed with 10% formalin for FFPE sections. a The protein abundance of COL1A2, VIM and activated LOX were measured by western blot. Representative blot images were shown. Different targets were run on the same membrane: COL1A2, VIM and activated LOX were probed in the same membrane and β-ACTIN was used as an endogenous control (n = 5–6 mice per group). b The localizations of COL1A1 and LOX were determined by

Article Snippet: Compared to the control groups, the protein abundanceof REV-ERBαwas decreased in the healthy area (Control: 21.294%vs. healthy area from IPF: 11.296%) Fig. 1 | Decreased REV-ERBα protein abundance and increasedprotein levels of COL1A1 and LOX in IPF lungs compared to healthy control.Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc.

Techniques: Infection, Isolation, Quantitative Proteomics, Western Blot, Membrane, Control

Journal: The Journal of experimental medicine

Article Title: A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes.

doi: 10.1084/jem.189.6.949

Figure Lengend Snippet: Figure 1. Expression of a gene identified by differential display. (A) Northern blot showing differential expression of an RNA species in Fas-sensitive primary B cells stimu- lated by CD40L alone (2) and in Fas-resistant B cells stimulated by CD40L plus anti-Ig (1), as de- scribed in Materials and Methods. Arrows indicate the location of ri- bosomal RNA. (B) Northern blot showing expression of an RNA species in various murine tissues, as indicated in figure.

Article Snippet: Initial Northern probing to confirm differential expression was performed as above except that differential display primers were used and the annealing step of PCR was performed for 2 min at 40 8 C. Autoradiography was performed using intensifying screens at 2 80 8 C for 1–3 d. A multiple tissue Northern blot was obtained from OriGene Technologies. cDNA Library Screening.

Techniques: Expressing, Northern Blot, Quantitative Proteomics