Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle.

doi: 10.1073/pnas.2402890121

Figure Lengend Snippet: Fig. 4. The t-tubule network develops in the absence of O-mannosylated α-DG. (A and B) Immunofluorescence of cryosections of ventricles from control and Pomt1 cKO mice to detect matriglycan (A and B) and β-DG (B). Ten µm cryosections were used in A. (Scale bar, 10 µm.) Sixteen µm cryosections were used in B. (Scale bar, 5 µm.) (C) FM 464-FX fluorescence of whole left ventricles to detect plasma membranes, including t-tubule membranes. (Scale bar, 20 µm.) (D) Quantification of the percent of myofibers with normal or disrupted t-tubule staining within a 90× magnification field. (E) Line scan analysis to determine the peak signal intensity of FM 464-FX labeled t-tubules (TT). (F) Number of t-tubules observed within 16 µm regions. Image analysis was performed on hearts of mice from both sexes (n = 4 controls; n = 5 cKO). Unpaired t tests with the Holm–Sidak post hoc test were performed. Data are expressed as mean ± SD.

Article Snippet: Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).

Techniques: Immunofluorescence, Control, Fluorescence, Clinical Proteomics, Staining, Labeling

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: PAR2 (Protease-Activated Receptor 2) Deficiency Attenuates Atherosclerosis in Mice

doi: 10.1161/atvbaha.117.310082

Figure Lengend Snippet: Figure 1. PAR2 (protease-activated receptor 2) mRNA and protein are increased in the atherosclerotic prone regions of mice and humans. Normal mouse aortic arch (low-density lipoprotein receptor–deficient [Ldlr−/−] mice fed a chow diet for 24 wk) or atherosclerosis-containing aortic arches (Ldlr−/− mice fed a Western diet for 24 wk) were examined for (A) mRNA extrapolated to normal and (B) protein expression (n=5 individual samples per group). Normal or diseased human carotid arteries were isolated and examined for (C) mRNA extrapolated to normal and (D) protein expression (n=5 individual samples per group). Immunologic detection of PAR2 expression was also performed on mouse atherosclerotic aortic sinus (E, F) and human carotid atherosclerotic lesions (H, I; n=4–5 individual samples per species; ×4 magnification E and H; ×10 magnification F and I). G, J, Quantification of PAR2 staining in the lesion or media of atherosclerotic lesions. Solid white box is zoomed in area. Dashed white line is the area of the lesion. Histobars represent mean±SEM. *P<0.05 for comparisons to normal aorta/artery or lesion vs tunica media (2-tailed Student t test).

Article Snippet: Immunostaining was performed on frozen serial sections as described previously.26 Human carotid atherosclerotic sections were obtained from Origene Technologies, Inc.

Techniques: Western Blot, Expressing, Isolation, Staining

Journal: Renal Failure

Article Title: miR-15a-5p suppresses peritoneal fibrosis induced by peritoneal dialysis via targeting VEGF in rats

doi: 10.1080/0886022x.2020.1811123

Figure Lengend Snippet: Figure 1. The rat uremia model was successfully constructed. (A) The serum of rats in each group was collected before periton- eal dialysis. The levels of creatinine (Scr) and blood urea nitrogen (BUN) are shown (p < 0.05). (B) Expression of miR-15a-5p and VEGF in the peritoneum, as detected by quantitative real-time PCR (qRT-PCR). p < 0.05 versus control, #p < 0.05 versus PD.

Article Snippet: Immunohistochemical staining of peritoneum tissue sections was performed using a rabbit two-step detection kit and a DAB developing kit (Goldenbridge Biotechnology Co. Ltd., Beijing, China).

Techniques: Construct, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control

Journal: Nature communications

Article Title: Circadian clock molecule REV-ERBα regulates lung fibrotic progression through collagen stabilization.

doi: 10.1038/s41467-023-36896-0

Figure Lengend Snippet: Fig. 1 | Decreased REV-ERBα protein abundance and increased protein levels of COL1A1 and LOX in IPF lungs compared to healthy control. Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc. Healthy controls contained 100% normal lung architecture with 85% alveoli surface area. IPF patient samples contained at least 50% lesion surface area. The protein abundance of REV-ERBα, COL1A1, and LOX were visualized and

Article Snippet: Compared to the control groups, the protein abundanceof REV-ERBαwas decreased in the healthy area (Control: 21.294%vs. healthy area from IPF: 11.296%) Fig. 1 | Decreased REV-ERBα protein abundance and increasedprotein levels of COL1A1 and LOX in IPF lungs compared to healthy control.Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc.

Techniques: Quantitative Proteomics, Control

Journal: Nature communications

Article Title: Circadian clock molecule REV-ERBα regulates lung fibrotic progression through collagen stabilization.

doi: 10.1038/s41467-023-36896-0

Figure Lengend Snippet: Fig. 7 | IAV infection induced dysregulation of profibrotic progression exa- cerbated in Rev-erbα Het mice. WT and Rev-erbα Het mice (equal number of male and female) infected (103 PFU/mouse) with IAV for 15 days, and lungs were sepa- rated for RNA/protein isolation, or fixed with 10% formalin for FFPE sections. a The protein abundance of COL1A2, VIM and activated LOX were measured by western blot. Representative blot images were shown. Different targets were run on the same membrane: COL1A2, VIM and activated LOX were probed in the same membrane and β-ACTIN was used as an endogenous control (n = 5–6 mice per group). b The localizations of COL1A1 and LOX were determined by

Article Snippet: Compared to the control groups, the protein abundanceof REV-ERBαwas decreased in the healthy area (Control: 21.294%vs. healthy area from IPF: 11.296%) Fig. 1 | Decreased REV-ERBα protein abundance and increasedprotein levels of COL1A1 and LOX in IPF lungs compared to healthy control.Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc.

Techniques: Infection, Isolation, Quantitative Proteomics, Western Blot, Membrane, Control